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2 Hours Assay Time CATHL1 ELISA Assay Kit / Human Cathelicidin-1 Sandwich ELISA Kit

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2 Hours Assay Time CATHL1 ELISA Assay Kit / Human Cathelicidin-1 Sandwich ELISA Kit

2 Hours Assay Time CATHL1 ELISA Assay Kit / Human Cathelicidin-1 Sandwich ELISA Kit
2 Hours Assay Time CATHL1 ELISA Assay Kit / Human Cathelicidin-1 Sandwich ELISA Kit

Large Image :  2 Hours Assay Time CATHL1 ELISA Assay Kit / Human Cathelicidin-1 Sandwich ELISA Kit

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E3541Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Bulk Order: Yes
Delivery: Within 48 Hours Target Protein: Cathelicidin-1
Test Method: Sandwich Size: 96 Wells/48 Wells
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2 Hours Assay Time CATHL1 ELISA Assay Kit Human Cathelicidin-1 Sandwich ELISA Kit For Research

 

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

 

Assay Principle

This Sandwich ELISA Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CATHL1 antibody. CATHL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CATHL1 Antibody is added and binds to CATHL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CATHL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CATHL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Cat.No E3541Hu

Sensitivity: 3.21ng/ml

Standard Curve Range: 7ng/ml - 300ng/ml

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Human Cathelicidin-1 (also known as CATHL1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (400ng/ml) with 120μl of standard diluent to generate a 200ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (200ng/ml) 1:2 with standard diluent to produce 100ng/ml, 50ng/ml, 25ng/ml and 12.5ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

200ng/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
100ng/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
50ng/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
25ng/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
12.5ng/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
400ng/ml 200ng/ml 100ng/ml 50ng/ml 25ng/ml 12.5ng/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

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Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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