Home
Products
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
Company News
Home ProductsELISA Test Kit

48 / 96 Wells FPR2 Mouse Sandwich Immunosorbent Assay Kit For Accurate Quantitative Detection

China Shanghai Korain Biotech Co., Ltd certification
China Shanghai Korain Biotech Co., Ltd certification
Providing high quality products、perfect after-sales service, and try best to meet the requirement of customers. We are very willing to continue long-term cooperation.

—— Tommy

We have confidence in working with you to expand the market one more year.

—— Lilis

I'm Online Chat Now

48 / 96 Wells FPR2 Mouse Sandwich Immunosorbent Assay Kit For Accurate Quantitative Detection

48 / 96 Wells FPR2 Mouse Sandwich Immunosorbent Assay Kit For Accurate Quantitative Detection
48 / 96 Wells FPR2 Mouse Sandwich Immunosorbent Assay Kit For Accurate Quantitative Detection

Large Image :  48 / 96 Wells FPR2 Mouse Sandwich Immunosorbent Assay Kit For Accurate Quantitative Detection

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E2622Mo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Quality: CE, ISO Known As: FPR2
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Assay Time: 2 Hours
Target Protein: Formyl Peptide Receptor 2 Bulk Order: Yes
High Light:

elisa assay kit

,

elisa reagent kit

48 / 96 Wells FPR2 Mouse Sandwich Immunosorbent Assay Kit For Accurate Quantitative Detection

 

Cat.No E2622Mo

Standard Curve Range: 20ng/L - 6000ng/L

Sensitivity: 11.86ng/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precautions

  • Prior to use, the elisa kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The elisa kit should not be used beyond the expiration date.

 

Assay Principle

This elisa kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse FPR2 antibody. FPR2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FPR2 Antibody is added and binds to FPR2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FPR2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FPR2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (6400ng/L) with 120μl of standard diluent to generate a 3200ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (3200ng/L) 1:2 with standard diluent to produce 1600ng/L, 800ng/L, 400ng/L and 200ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

3200ng/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
1600ng/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
800ng/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
400ng/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
200ng/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
6400ng/L 3200ng/L 1600ng/L 800ng/L 400ng/L 200ng/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

Send your inquiry directly to us (0 / 3000)