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48 Wells Chicken SOD Enzyme Linked Immunosorbent Assay Elisa 2 Hours Assay Length

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48 Wells Chicken SOD Enzyme Linked Immunosorbent Assay Elisa 2 Hours Assay Length

48 Wells Chicken SOD Enzyme Linked Immunosorbent Assay Elisa 2 Hours Assay Length
48 Wells Chicken SOD Enzyme Linked Immunosorbent Assay Elisa 2 Hours Assay Length

Large Image :  48 Wells Chicken SOD Enzyme Linked Immunosorbent Assay Elisa 2 Hours Assay Length

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0011Ch

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
OEM: Acceptable Quality: CE, ISO
Organism Species: Chicken Delivery: Within 48 Hours
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Shipping: DHL/FedEX
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elisa assay kit

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elisa kit

96 Wells 48 Wells Chicken SOD Enzyme Linked Immunosorbent ELISA Kit 2 Hours Assay Length
 

Cat.No E0011Ch

Standard Curve Range: 3U/L - 900U/L

Sensitivity: 1.56U/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Chicken Super Oxidase Dimutase (also known as SOD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Reagent Provided

Components Quantity
Standard Solution (960U/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Chicken SOD Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

 

ELISA Kit Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (960U/L) with 120μl of standard diluent to generate a 480U/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (480U/L) 1:2 with standard diluent to produce 240U/L, 120U/L, 60U/L and 30U/L solutions. Standard diluent serves as the zero standard(0 U/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

480U/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
240U/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
120U/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
60U/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
30U/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
960U/L 480U/L 240U/L 120U/L 60U/L 30U/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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