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96 Wells ELISA Test Kit 0.28ng/L Sensitivity For Human Cardiac Troponin T Test

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96 Wells ELISA Test Kit 0.28ng/L Sensitivity For Human Cardiac Troponin T Test

96 Wells ELISA Test Kit 0.28ng/L Sensitivity For Human Cardiac Troponin T Test
96 Wells ELISA Test Kit 0.28ng/L Sensitivity For Human Cardiac Troponin T Test

Large Image :  96 Wells ELISA Test Kit 0.28ng/L Sensitivity For Human Cardiac Troponin T Test

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E4862Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Custom: Available Storage: 2-8°C
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Assay Length: 2 Hours
Shipping: DHL/FedEX Target Protein: High Sensitivity Cardiac Troponin T
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elisa reagent kit

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High Sensitive hs-cTnT Immunoassays Test Kit High Sensitivity Cardiac Troponin T ELISA Assay Kit

 

Cat.No E4862Hu

Size: 96 wells

Sensitivity: 0.28ng/L

Standard Curve Range: 0.5ng/L - 300ng/L

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue other body fluids Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of human High Sensitivity Cardiac Troponin T (also known as hs-cTnT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This ELISA Assay Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human hs-cTnT antibody. hs-cTnT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human hs-cTnT Antibody is added and binds to hs-cTnT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated hs-cTnT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human hs-cTnT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (320ng/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated human hs-cTnT Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 

Precautions

  • Prior to use, the ELISA Assay Kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The ELISA Assay Kit should not be used beyond the expiration date.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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