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96 Wells Rat GAL3 ELISA Test Kit Under 2 - 8°C Storage Condition

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96 Wells Rat GAL3 ELISA Test Kit Under 2 - 8°C Storage Condition

96 Wells Rat GAL3 ELISA Test Kit Under 2 - 8°C Storage Condition
96 Wells Rat GAL3 ELISA Test Kit Under 2 - 8°C Storage Condition

Large Image :  96 Wells Rat GAL3 ELISA Test Kit Under 2 - 8°C Storage Condition

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E1533Ra

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Cat.No: E1533Ra Target Protein: Galectin-3
Lead Time: Within 48 Hours Shipping: DHL/FedEX
Uniprot No.: P08699 Organism Species: Rat
High Light:

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immunoassay test kits

96 wells Rat GAL3 ELISA Kit High specificity and precision

 

Cat.No E1533Ra

Standard Curve Range: 0.05ng/ml - 20ng/ml

Sensitivity: 0.023ng/ml

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Rat Galectin 3 (also known as GAL-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat GAL-3 antibody. GAL-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GAL-3 Antibody is added and binds to GAL-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAL-3antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GAL-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (24ng/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Rat GAL-3 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (24ng/ml) with 120μl of standard diluent to generate a 12ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (12ng/ml) 1:2 with standard diluent to produce 6ng/ml, 3ng/ml, 1.5ng/ml and 0.75ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

12ng/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
6ng/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
3ng/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
1.5ng/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
0.75ng/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
24ng/ml 12ng/ml 6ng/ml 3ng/ml 1.5ng/ml 0.75ng/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

  1. Prepare all reagents, samples and standards.
  2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
  3. Wash the plate 5 times.

  4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

  5. Add stop solution and color develops.

  6. Read the OD value within 10 minutes.

 

References

Bellac C.L., Coimbra R.S., Simon F., Imboden H., Leib S.L.
Neurobiol. Dis. 28:175-183(2007)

 
 

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Contact Person: Lee

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