Accurate Goat Anti Rabbit Secondary Antibody Rhodamine TRITC 0.1 mg/ml Concentration

Goat Anti-Rabbit IgG ( H+L ) Secondary Antibody, TRITC Conjugate

Cat.No. BT-AS00394 Size: 100ul Datasheet Request
Product Information
Clonallity: Polyclonal
Applications: IHC-P, IHC-F, ICC, WB
Source: Goat
Immunogen: whole molecule rabbit IgG
Concentration: 0.1 mg/ml
Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Purification:
The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.
Synonyms:
TRITC-conjugated Goat Anti-Rabbit IgG, Goat Anti-Rabbit IgG-TRITC Secondary Antibody, Rhodamine-labeled Goat Anti-Rabbit IgG Secondary Antibody
 

Recommended Dilution:
WB: 1 : 10000 - 1: 50000
IHC: 1 : 500 -1 : 5000
ICC: 1:20 - 1:100
IF: 1:20 - 1:100
FC: 1:20 - 1:100
ELISA: 1 : 10000 -1 : 50000
Not yet tested in other applications

Storage:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Note:

This product is for research use only,not for diagnostic or therapeutic use.

Immunofluorescence analysis of HeLa cells, using PARP1 antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.

Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) at 1:100 dilution.

Blue: DAPI for nuclear staining.