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Accurate Quantitative Detection Superoxide Dismutase Assay Kit 20ng/L - 3800ng/L Standard Curve Rang

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Accurate Quantitative Detection Superoxide Dismutase Assay Kit 20ng/L - 3800ng/L Standard Curve Rang

Accurate Quantitative Detection Superoxide Dismutase Assay Kit 20ng/L - 3800ng/L Standard Curve Rang
Accurate Quantitative Detection Superoxide Dismutase Assay Kit 20ng/L - 3800ng/L Standard Curve Rang

Large Image :  Accurate Quantitative Detection Superoxide Dismutase Assay Kit 20ng/L - 3800ng/L Standard Curve Rang

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0153Go

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Cat.No: E0153Go Standard Curve Rang: 20ng/L - 3800ng/L
Sensitivity: 11.03ng/L Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
Lead Time: Within 48 Hours Storage: 2-8°C
High Light:

elisa assay kit

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elisa kit

96 Well Plate Goat Customized Glutathione Peroxidase 2 Sandwich ELISA Kit 2 Hours Assay Length

 

Cat.No E0153Go

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (4000ng/L) with 120μl of standard diluent to generate a 2000ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (2000ng/L) 1:2 with standard diluent to produce 1000ng/L, 500ng/L, 250ng/L and 125ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

2000ng/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
1000ng/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
500ng/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
250ng/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
125ng/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
4000ng/L 2000ng/L 1000ng/L 500ng/L 250ng/L 125ng/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Goat Glutathione Peroxidase 2 (also known as GPX2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Reagent Provided

Components Quantity
Standard Solution (4000ng/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Goat GPX2 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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