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Cat.NO: | BT-AP00082 | Source: | Rabbit |
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Concentration: | 1 Mg/ml | Gene Symbol: | AKT1 |
Isotype: | IgG | Mol Wt: | 56 KDa |
High Light: | rabbit antibody,polyclonal antibodies |
PKB Rabbit Polyclonal Antibody
Cat.No. BT-AP00082 Size: 100ul/50ul/20ul Datasheet Request
Product Information
Clonallity: Polyclonal
Applications: WB IHC-p
Source: Rabbit
Immunogen: Synthesized peptide derived from human PDK1 around the non-phosphorylation site of Y9
Protein Name: 3-phosphoinositide-dependent protein kinase 1
Species reactivity: human, mouse, rat
Observed band: 60 kDa
Concentration: 1 mg/ml
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Purification:
The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.
Synonyms:
TNF-alpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF-a; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; Tumour Necrosis Factor Alpha; TNFSF1A; TNFA_HUMAN.
Recommended Dilution:
WB: 1: 500 - 1: 2000
IHC: 1: 100 - 1: 300
ELISA: 1: 40000
Not yet tested in other applications
Storage:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.
Background
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Contact Person: Lee
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