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Bovine High Specificity NEFA Sandwich Immunoassay Kit For Accurate Quantitative Detection

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Bovine High Specificity NEFA Sandwich Immunoassay Kit For Accurate Quantitative Detection

Bovine High Specificity NEFA Sandwich Immunoassay Kit For Accurate Quantitative Detection
Bovine High Specificity NEFA Sandwich Immunoassay Kit For Accurate Quantitative Detection

Large Image :  Bovine High Specificity NEFA Sandwich Immunoassay Kit For Accurate Quantitative Detection

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2015, MSDS
Model Number: Cat.No E0021Bo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Quality: CE, ISO Target Protein: Non-esterified Fatty Acid
Storage: 2-8°C Test Method: Sandwich
Delivery: Within 48 Hours Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
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Bovine High Specificity NEFA Sandwich Immunoassay Kit For Accurate Quantitative Detection
 

Cat.No E0021Bo

Standard Curve Range: 2μmol/L - 600μmol/L

Sensitivity: 1.17μmol/L

Size: 96 wells

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Bovine Non-esterified Fatty Acid (also known as NEFA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

 

Reagent Provided

Components Quantity
Standard Solution (640μmol/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Bovine NEFA Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 

Precautions

  • Prior to use, the ELISA Assay Kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (640μmol/L) with 120μl of standard diluent to generate a 320μmol/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (320μmol/L) 1:2 with standard diluent to produce 160μmol/L, 80μmol/L, 40μmol/L and 20μmol/L solutions. Standard diluent serves as the zero standard(0 μmol/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

320μmol/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
160μmol/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
80μmol/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
40μmol/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
20μmol/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
640μmol/L 320μmol/L 160μmol/L 80μmol/L 40μmol/L 20μmol/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 600 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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