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Bovine SDH Sandwich ELISA Kit Strong Sensitivity Sorbitol Dehydrogenase ELISA Test Kit

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Bovine SDH Sandwich ELISA Kit Strong Sensitivity Sorbitol Dehydrogenase ELISA Test Kit

Bovine SDH Sandwich ELISA Kit Strong Sensitivity Sorbitol Dehydrogenase ELISA Test Kit
Bovine SDH Sandwich ELISA Kit Strong Sensitivity Sorbitol Dehydrogenase ELISA Test Kit

Large Image :  Bovine SDH Sandwich ELISA Kit Strong Sensitivity Sorbitol Dehydrogenase ELISA Test Kit

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2015, MSDS
Model Number: Cat.No E0079Bo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Storage: 2-8°C Sensitivity: 0.27mIU/ml
Standard Curve Range: 0.5mIU/ml - 100mIU/ml Shipping: DHL/FedEX
Discount: Available Size: 96 Wells/48 Wells
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Bovine SDH Sandwich ELISA Kit Strong Sensitivity Sorbitol Dehydrogenase ELISA Test Kit
 

Cat.No E0079Bo

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Bovine Sorbitol Dehydrogenase (also known as SDH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This Sandwich ELISA Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine SDH antibody. SDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine SDH Antibody is added and binds to SDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine SDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (128mIU/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Bovine SDH Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Note

  • Sample concentrations should be predicted before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their particular experiments.
  • Samples to be used within 5 days should be stored at 2-8°C. Samples should be aliquoted or must be stored at -20°C within 1 month or -80°C within 6 months. Avoid repeated freeze thaw cycles.
  • Samples should be brought to room temperature before starting the assay.
  • Centrifuge to collect sample before use.
  • Samples containing NaN3 can’t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).
  • Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
  • Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.

*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (128mIU/ml) with 120μl of standard diluent to generate a 64mIU/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (64mIU/ml) 1:2 with standard diluent to produce 32mIU/ml, 16mIU/ml, 8mIU/ml and 4mIU/ml solutions. Standard diluent serves as the zero standard(0 mIU/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

64mIU/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
32mIU/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
16mIU/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
8mIU/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
4mIU/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
128mIU/ml 64mIU/ml 32mIU/ml 16mIU/ml 8mIU/ml 4mIU/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 600 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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