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CE Certification Elisa Assay Kit 1ng / Ml - 400ng / Ml Standard Curve Range

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CE Certification Elisa Assay Kit 1ng / Ml - 400ng / Ml Standard Curve Range

CE Certification Elisa Assay Kit 1ng / Ml - 400ng / Ml Standard Curve Range
CE Certification Elisa Assay Kit 1ng / Ml - 400ng / Ml Standard Curve Range

Large Image :  CE Certification Elisa Assay Kit 1ng / Ml - 400ng / Ml Standard Curve Range

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0166Ra

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Quality: CE, ISO Standard Curve Range: 1ng/ml - 400ng/ml
Sensitivity: 0.52ng/ml Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
Assay Time: 2 Hours Delivery: Within 48 Hours
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96 Wells 48 Wells Rat IFABP ELISA Kit Strong Sensitivity For Accurate Quantitative Detection
 
Cat.No E0166Ra
Standard Curve Range: 1ng/ml - 400ng/ml
Sensitivity: 0.52ng/ml
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
 
Intended Use
This sandwich kit is for the accurate quantitative detection of Rat Intestinal Fatty Acid Binding Protein (also known as IFABP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
 
Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat IFABP antibody. IFABP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IFABP Antibody is added and binds to IFABP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFABP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat IFABP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Reagent Provided

ComponentsQuantity
Standard Solution (480ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Rat IFABP Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (480ng/ml) with 120μl of standard diluent to generate a 240ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (240ng/ml) 1:2 with standard diluent to produce 120ng/ml, 60ng/ml, 30ng/ml and 15ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
 

240ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
120ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
60ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
30ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
15ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
480ng/ml240ng/ml120ng/ml60ng/ml30ng/ml15ng/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.
 
Calculation of Result
Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.
 
Referances
"Chronic leptin administration decreases fatty acid uptake and fatty acid transporters in rat skeletal muscle."
Steinberg G.R., Dyck D.J., Calles-Escandon J., Tandon N.N., Luiken J.J., Glatz J.F., Bonen A.
J. Biol. Chem. 277:8854-8860(2002)

Contact Details
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Contact Person: Lee

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