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Customized Canine MCP-1 CCL2 Strong Sensitivity MCAF ELISA Test Kit With 2 Hours Assay Time

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Customized Canine MCP-1 CCL2 Strong Sensitivity MCAF ELISA Test Kit With 2 Hours Assay Time

Customized Canine MCP-1 CCL2 Strong Sensitivity MCAF ELISA Test Kit With 2 Hours Assay Time
Customized Canine MCP-1 CCL2 Strong Sensitivity MCAF ELISA Test Kit With 2 Hours Assay Time

Large Image :  Customized Canine MCP-1 CCL2 Strong Sensitivity MCAF ELISA Test Kit With 2 Hours Assay Time

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0135Ca

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Size: 96 Wells/48 Wells Storage: 2-8°C
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Assay Length: 2 Hours
Lead Time: Within 48 Hours Target Protein: Monocyte Chemotactic Protein 1
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Customized Canine MCP-1 CCL2 Strong Sensitivity MCAF ELISA Test Kit With 2 Hours Assay Time
 
Assay Principle
This ELISA kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine MCP-1/CCL2/MCAF antibody. MCP-1/CCL2/MCAF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine MCP-1/CCL2/MCAF Antibody is added and binds to MCP-1/CCL2/MCAF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MCP-1/CCL2/MCAF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine MCP-1/CCL2/MCAF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Intended Use
 
This sandwich kit is for the accurate quantitative detection of Canine Monocyte chemotactic protein 1,monocyte chemotactic and activating factor (also known as MCP-1/CCL2/MCAF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
 
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
 
Reagent Provided

ComponentsQuantity
Standard Solution (1280ng/L)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (25x)20ml x1
Biotinylated Canine MCP-1/CCL2/MCAF Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Precautions

  • Prior to use, the ELISA kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (1280ng/L) with 120μl of standard diluent to generate a 640ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (640ng/L) 1:2 with standard diluent to produce 320ng/L, 160ng/L, 80ng/L and 40ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
 

640ng/LStandard No.5120μl Original Standard + 120μl Standard Diluent
320ng/LStandard No.4120μl Standard No.5 + 120μl Standard Diluent
160ng/LStandard No.3120μl Standard No.4 + 120μl Standard Diluent
80ng/LStandard No.2120μl Standard No.3 + 120μl Standard Diluent
40ng/LStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
1280ng/L640ng/L320ng/L160ng/L80ng/L40ng/L

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.
 
Cat.No E0135Ca
Standard Curve Range: 5ng/L - 1000ng/L
Sensitivity: 2.55ng/L

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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