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Customized NTRK2 ELISA Test Kit Human BDNF/NT-3 Growth Factors Receptor Immunoassay Lab Test

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Customized NTRK2 ELISA Test Kit Human BDNF/NT-3 Growth Factors Receptor Immunoassay Lab Test

Customized NTRK2 ELISA Test Kit Human BDNF/NT-3 Growth Factors Receptor Immunoassay Lab Test
Customized NTRK2 ELISA Test Kit Human BDNF/NT-3 Growth Factors Receptor Immunoassay Lab Test

Large Image :  Customized NTRK2 ELISA Test Kit Human BDNF/NT-3 Growth Factors Receptor Immunoassay Lab Test

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0012Ha

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Bulk Order: Yes Discount: Available
Standard Curve Range: 0.3ng/ml - 90ng/ml Sensitivity: 0.13ng/ml
Customized: Acceptable Target Protein: BDNF/NT-3 Growth Factors Receptor
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elisa assay kit

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elisa kit

Customized NTRK2 ELISA Assay Kit Human BDNF/NT-3 Growth Factors Receptor ELISA Test Kit

 

Cat.No E6633Hu

Size: Customized NTRK2 Immunoassay Lab Test Human BDNF/NT-3 Growth Factors Receptor 96 Wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Reagent Provided

Components Quantity
Standard Solution (96ng/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Human NTRK2 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Precautions

  • Prior to use, the elisa assay kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The elisa test kit should not be used beyond the expiration date.

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue and other body fluids Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of human BDNF/NT-3 Growth Factors Receptor (also known as NTRK2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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