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D - Dimer Human Elisa Kit High Specificity For Accurate Quantitative Detection

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D - Dimer Human Elisa Kit High Specificity For Accurate Quantitative Detection

D - Dimer Human Elisa Kit High Specificity For Accurate Quantitative Detection
D - Dimer Human Elisa Kit High Specificity For Accurate Quantitative Detection

Large Image :  D - Dimer Human Elisa Kit High Specificity For Accurate Quantitative Detection

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E1004Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Customized: Acceptable Cat.No: E1004Hu
Target Protein: D-Dimer Shipping: DHL/FedEX
OEM: Acceptable Storage: 2-8°C
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
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enzyme assay kits

High Specificity Human D-Dimer ELISA Kit for Accurate Quantitative Detection

 

Cat.No E1113Hu

Standard Curve Range: 20pg/ml - 4000pg/ml

Sensitivity: 10.53pg/ml

Size: 96 wells

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

Reagent Provided

Components Quantity
Standard Solution (4800pg/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated human D2D Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (4800pg/ml) with 120μl of standard diluent to generate a 2400pg/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (2400pg/ml) 1:2 with standard diluent to produce 1200pg/ml, 600pg/ml, 300pg/ml and 150pg/ml solutions. Standard diluent serves as the zero standard(0 pg/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

2400pg/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
1200pg/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
600pg/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
300pg/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
150pg/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
4800pg/ml 2400pg/ml 1200pg/ml 600pg/ml 300pg/ml 150pg/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

 

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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