Escherichia Coli Recombinant Protein Aeromonas Aminopeptidase for Research Use

Highly sensitive and specific Recombinant Aeromonas Aminopeptidase Protein

Catalog NO. CD0423

Synonyms: Aeromonas Aminopeptidase, Recombinant

Size: 100ug

Storage: -20℃ Datasheet Request

Molecular Weight: 31.4 kDa

Purity

Protein is > 98 % by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2μmfiltered concentrated solution in 20 mM Tris, 150 mM NaCl, 5μM ZnSO4, pH 8.0.

Biological Activity:

Sequentially cleaves N-terminal amino acids except E, D, and X-P.

Endotoxin:

Less than 0.1 EU/μg of rAeromonas Aminopeptidase as determined by LAL method.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Notes:

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Background

Aminopeptidases catalyze the cleavage of amino acids from the amino terminus of protein or peptide substrates. They are widely distributed throughout the animal and plant kingdoms and are found in many subcellular organelles, in cytoplasm and as membrane components. Some aminopeptidases are monomeric and others are assemblies of relatively high mass (50 kDa) subunits. Aminopeptidases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Recombinant Aeromonas Aminopeptidase is a 31.4 kDa protein containing 291 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.