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|Concentration:||0.1 Mg/ml||Immunogen:||Whole Molecule Rabbit IgG|
|Brand:||BT Lab||Applications:||IHC-P, IHC-F, ICC, WB|
elisa secondary antibody,
Goat Anti-Rabbit IgG ( H+L ) Secondary Antibody
Cat.No. BT-AS00394 Size: 100ul Datasheet Request
Applications: IHC-P, IHC-F, ICC, WB
Immunogen: whole molecule rabbit IgG
Concentration: 0.1 mg/ml
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.
TRITC-conjugated Goat Anti-Rabbit IgG, Goat Anti-Rabbit IgG-TRITC Secondary Antibody, Rhodamine-labeled Goat Anti-Rabbit IgG Secondary Antibody
WB: 1 : 10000 - 1: 50000
IHC: 1 : 500 -1 : 5000
ICC: 1:20 - 1:100
IF: 1:20 - 1:100
FC: 1:20 - 1:100
ELISA: 1 : 10000 -1 : 50000
Not yet tested in other applications
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.
Immunofluorescence analysis of HeLa cells, using antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) at 1:200 dilution.
Blue: DAPI for nuclear staining.
Contact Person: Lee