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Guinea Pig Antibody ELISA Test Kit , 96 Wells Interleukin 2 ELISA Kit

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Guinea Pig Antibody ELISA Test Kit , 96 Wells Interleukin 2 ELISA Kit

Guinea Pig Antibody ELISA Test Kit , 96 Wells Interleukin 2  ELISA Kit
Guinea Pig Antibody ELISA Test Kit , 96 Wells Interleukin 2  ELISA Kit

Large Image :  Guinea Pig Antibody ELISA Test Kit , 96 Wells Interleukin 2 ELISA Kit

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0069Gp

Payment & Shipping Terms:

Minimum Order Quantity: 1 kit
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
Detailed Product Description
Target Protein: Interleukin 2 Sample: 5.68ng/L
Sensitivity: 2 Hours Discount: Available
Lead Time: Within 48 Hours Assay Principle: Sandwich
High Light:

elisa reagent kit

,

elisa kit

Guinea Pig Interleukin 2 ELISA Kit with High Spesificity and Sensitivity 

Cat.No E0069Gp

Standard Curve Range: 10ng/L - 2000ng/L

Sensitivity: 5.68ng/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Guinea Pig Interleukin 2 (also known as IL-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Guinea Pig IL-2 antibody. IL-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Guinea Pig IL-2 Antibody is added and binds to IL-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Guinea Pig IL-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (2400ng/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Guinea Pig IL-2 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (2400ng/L) with 120μl of standard diluent to generate a 1200ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (1200ng/L) 1:2 with standard diluent to produce 600ng/L, 300ng/L, 150ng/L and 75ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

 

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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