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High Precision Sensitivity ELISA Assay Kit For Research Mouse Phospho Insulin Receptor Beta

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High Precision Sensitivity ELISA Assay Kit For Research Mouse Phospho Insulin Receptor Beta

High Precision Sensitivity ELISA Assay Kit For Research Mouse Phospho Insulin Receptor Beta
High Precision Sensitivity ELISA Assay Kit For Research Mouse Phospho Insulin Receptor Beta

Large Image :  High Precision Sensitivity ELISA Assay Kit For Research Mouse Phospho Insulin Receptor Beta

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E2652Mo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Size: 96 Wells/48 Wells Shipping: DHL/FedEX
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant OEM: Acceptable
Storage: 2-8°C Discount: Available
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High Precision and Sensitivity Mouse Phospho-Insulin Receptor Beta ELISA Assay Kit For Research
 

Cat.No E2652Mo

Standard Curve Range: 10ng/L - 2000ng/L

Sensitivity: 5.08ng/L

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Mouse Phospho-Insulin Receptor Beta (Tyr1162,Tyr1163) (also known as p-ISR-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (2400ng/L) with 120μl of standard diluent to generate a 1200ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (1200ng/L) 1:2 with standard diluent to produce 600ng/L, 300ng/L, 150ng/L and 75ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

1200ng/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
600ng/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
300ng/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
150ng/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
75ng/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
2400ng/L 1200ng/L 600ng/L 300ng/L 150ng/L 75ng/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

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Contact Person: Lee

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