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|Target Protein:||Interferon Alpha||Sample:||Serum,plasma,urine,tissue,cell Culture Supernatant|
|Assay Time:||2 Hours||Standard Curve Range:||1ng/L - 400ng/L|
elisa reagent kit,
Cat Interferon Alpha ELISA Kit with High Spesificity and Sensitivity
This sandwich kit is for the accurate quantitative detection of Cat Interferon Alpha (also known as IFN-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Cat IFN-A antibody. IFN-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Cat IFN-A Antibody is added and binds to IFN-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFN-A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Cat IFN-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
|Standard Solution (480ng/L)||0.5ml x1|
|Pre-coated ELISA Plate||12 * 8 well strips x1|
|Standard Diluent||3ml x1|
|Stop Solution||6ml x1|
|Substrate Solution A||6ml x1|
|Substrate Solution B||6ml x1|
|Wash Buffer Concentrate (30x)||20ml x1|
|Biotinylated Cat IFN-A Antibody||1ml x1|
|Plate Sealer||2 pics|
|Zipper bag||1 pic|
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
Contact Person: Lee