Payment & Shipping Terms:
|Target Protein:||Leptin||Uniprot No.:||P41159|
|Assay Time:||2 Hours||Known As:||LEP|
|Customized:||Acceptable||Lead Time:||Within 48 Hours|
enzyme assay kits,
96 wells High Sensitive ELISA Kit for Human Leptin
Standard Curve Range: 0.05ng/ml - 10ng/ml
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
This sandwich kit is for the accurate quantitative detection of human Leptin (also known as LEP) in serum, plasma, cell
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
Cassano S., Pucino V., La Rocca C., Procaccini C., De Rosa V., Marone G., Matarese G.
Contact Person: Lee