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Immunoglobulin M Porcine ELISA Kit / IgM ELISA Kit with Sandwich Assay Principle

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Immunoglobulin M Porcine ELISA Kit / IgM ELISA Kit with Sandwich Assay Principle

Immunoglobulin M Porcine ELISA Kit / IgM ELISA Kit with Sandwich Assay Principle
Immunoglobulin M Porcine ELISA Kit / IgM ELISA Kit with Sandwich Assay Principle

Large Image :  Immunoglobulin M Porcine ELISA Kit / IgM ELISA Kit with Sandwich Assay Principle

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0229Po

Payment & Shipping Terms:

Minimum Order Quantity: 1 kit
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
Detailed Product Description
Bulk Order: Immunoglobulin M Quality: CE, ISO
Assay Time: 2 Hours Discount: Available
Customized: Acceptable Assay Principle: Sandwich
High Light:

protein elisa kit

,

elisa test kit

Porcine Immunoglobulin M ELISA Kit with High Spesificity and Sensitivity 

Cat.No E0229Po

Standard Curve Range: 0.05mg/ml - 20mg/ml

Sensitivity: 0.022mg/ml

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Porcine Immunoglobulin M (also known as IgM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IgM antibody. IgM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IgM Antibody is added and binds to IgM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IgM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IgM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity

Standard Solution (24mg/ml)

0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Porcine IgM Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (24mg/ml) with 120μl of standard diluent to generate a 12mg/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (12mg/ml) 1:2 with standard diluent to produce 6mg/ml, 3mg/ml, 1.5mg/ml and 0.75mg/ml solutions. Standard diluent serves as the zero standard(0 mg/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

 

 

 

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Contact Person: Lee

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