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Laboratory Research Human ABCA1 Immunoassay Test Kit With High Sensitivity

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Laboratory Research Human ABCA1 Immunoassay Test Kit With High Sensitivity

Laboratory Research Human ABCA1 Immunoassay Test Kit With High Sensitivity
Laboratory Research Human ABCA1 Immunoassay Test Kit With High Sensitivity

Large Image :  Laboratory Research Human ABCA1 Immunoassay Test Kit With High Sensitivity

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E1483Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Size: 96 Wells/48 Wells OEM: Acceptable
Shipping: DHL/FedEX Lead Time: Within 48 Hours
Customized: Acceptable Known As: ABCA1
High Light:

enzyme immunoassay kit

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enzyme assay kits

Laboratory Research Human ABCA1 Immunoassays Test Kit With High Sensitivity and Specificity

 

Cat.No E1483Hu

 

Assay Principle

This Immunoassays Test Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ABCA1 antibody. ABCA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ABCA1 Antibody is added and binds to ABCA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ABCA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Note

  • Sample concentrations should be predicted before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their particular experiments.
  • Samples to be used within 5 days should be stored at 2-8°C. Samples should be aliquoted or must be stored at -20°C within 1 month or -80°C within 6 months. Avoid repeated freeze thaw cycles.
  • Samples should be brought to room temperature before starting the assay.
  • Centrifuge to collect sample before use.
  • Samples containing NaN3 can’t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).
  • Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
  • Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.

*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.

Reagent Provided

Components Quantity
Standard Solution (32ng/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Human ABCA1 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Standard Curve Range: 0.05ng/ml - 30ng/ml

Sensitivity: 0.029ng/ml

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precautions

  • Prior to use, the Immunoassays Test Kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The Immunoassays Test Kit should not be used beyond the expiration date.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (32ng/ml) with 120μl of standard diluent to generate a 16ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (16ng/ml) 1:2 with standard diluent to produce 8ng/ml, 4ng/ml, 2ng/ml and 1ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

16ng/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
8ng/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
4ng/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
2ng/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
1ng/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
32ng/ml 16ng/ml 8ng/ml 4ng/ml 2ng/ml 1ng/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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