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Laboratory Research Use Rat ELISA Kit Pre - Coated with Rat GHRL Antibody

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Laboratory Research Use Rat ELISA Kit Pre - Coated with Rat GHRL Antibody

Laboratory Research Use Rat ELISA Kit Pre - Coated with Rat GHRL Antibody
Laboratory Research Use Rat ELISA Kit Pre - Coated with Rat GHRL Antibody

Large Image :  Laboratory Research Use Rat ELISA Kit Pre - Coated with Rat GHRL Antibody

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0896Ra

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Uniprot No.: Q5Y391 Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
Assay Length: 2 Hours Known As: GHRL
Brand: BT Lab Delivery: Within 48 Hours
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enzyme assay kits

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immunoassay test kits

Specificity and sensitivity Rat Ghrelin ELISA Kit 96 wells

 

Cat.No E0896Ra

Standard Curve Range: 40ng/L - 12000ng/L

Sensitivity: 20.56ng/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Rat Ghrelin (also known as GHRL) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat GHRL antibody. GHRL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GHRL Antibody is added and binds to GHRL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GHRL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GHRL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (12800ng/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Rat GHRL Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (12800ng/L) with 120μl of standard diluent to generate a 6400ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (6400ng/L) 1:2 with standard diluent to produce 3200ng/L, 1600ng/L, 800ng/L and 400ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

6400ng/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
3200ng/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
1600ng/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
800ng/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
400ng/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
12800ng/L 6400ng/L 3200ng/L 1600ng/L 800ng/L 400ng/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

 

References

Ariyasu H., Takaya K., Hosoda H., Iwakura H., Ebihara K., Mori K., Ogawa Y., Hosoda K., Akamizu T., Kojima M., Kangawa K., Nakao K.
Endocrinology 143:3341-3350(2002)

 
 

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