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Msds Approved Enzyme Immunoassay Kit , Kidney Injury Molecule 1 Elisa Assay Kit

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Msds Approved Enzyme Immunoassay Kit , Kidney Injury Molecule 1 Elisa Assay Kit

Msds Approved Enzyme Immunoassay Kit , Kidney Injury Molecule 1 Elisa Assay Kit
Msds Approved Enzyme Immunoassay Kit , Kidney Injury Molecule 1 Elisa Assay Kit Msds Approved Enzyme Immunoassay Kit , Kidney Injury Molecule 1 Elisa Assay Kit

Large Image :  Msds Approved Enzyme Immunoassay Kit , Kidney Injury Molecule 1 Elisa Assay Kit

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E1099Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Test Method: Sandwich Target Protein: Kidney Injury Molecule 1
Uniprot No.: Q96D42 Organism Species: Human
Delivery: Within 48 Hours Storage: 2-8°C
High Light:

enzyme immunoassay kit

,

enzyme assay kits

96 Wells Human High Specificity Kidney Injury Molecule 1 ELISA Kit Customized
 
Cat.No E1099Hu

Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Kim-1 antibody. Kim-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Kim-1 Antibody is added and binds to Kim-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Kim-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Kim-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Reagent Provided

Components Quantity
Standard Solution (12.8ng/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated human KIM-1 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 
Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
 
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
 
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
 
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Note

  • Sample concentrations should be predicted before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their particular experiments.
  • Samples to be used within 5 days should be stored at 2-8°C. Samples should be aliquoted or must be stored at -20°C within 1 month or -80°C within 6 months. Avoid repeated freeze thaw cycles.
  • Samples should be brought to room temperature before starting the assay.
  • Centrifuge to collect sample before use.
  • Samples containing NaN3 can’t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).
  • Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
  • Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.

*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.

 

Calculation of Result
Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

 

Standard Curve Range: 0.05ng/ml - 10ng/ml
Sensitivity: 0.024ng/ml
Size: 96 wells
*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

References

"Immunoglobulin A (IgA) is a natural ligand of hepatitis A virus cellular receptor 1 (HAVCR1), and the association of IgA with HAVCR1 enhances virus-receptor interactions."

Tami C., Silberstein E., Manangeeswaran M., Freeman G.J., Umetsu S.E., DeKruyff R.H., Umetsu D.T., Kaplan G.G.
J. Virol. 81:3437-3446(2007)

 

Contact Details
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Contact Person: Lee

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