Home
Products
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
Company News
Home ProductsMouse ELISA Kit

No Sandwich Mouse Elisa Kit High Sensitivity Specificity 96 Wells / 48 Wells

China Shanghai Korain Biotech Co., Ltd certification
China Shanghai Korain Biotech Co., Ltd certification
Providing high quality products、perfect after-sales service, and try best to meet the requirement of customers. We are very willing to continue long-term cooperation.

—— Tommy

We have confidence in working with you to expand the market one more year.

—— Lilis

I'm Online Chat Now

No Sandwich Mouse Elisa Kit High Sensitivity Specificity 96 Wells / 48 Wells

No Sandwich Mouse Elisa Kit High Sensitivity Specificity 96 Wells / 48 Wells
No Sandwich Mouse Elisa Kit High Sensitivity Specificity 96 Wells / 48 Wells

Large Image :  No Sandwich Mouse Elisa Kit High Sensitivity Specificity 96 Wells / 48 Wells

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0439Mo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
Detailed Product Description
OEM: Acceptable Assay Length: 2 Hours
Discount: Available Shipping: DHL/FedEX
Storage: 2-8°C Organism Species: Mouse
High Light:

rat elisa kit

,

sandwich elisa kit

96 Wells 48 Wells Mouse Strong Specificity and Sensitivity NO Sandwich ELISA Kit
​​

Cat.No E0439Mo

Standard Curve Range: 1μmol/L - 400μmol/L

Sensitivity: 0.51μmol/L

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Mouse Nitric Oxide (also known as NO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse NO antibody. NO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NO Antibody is added and binds to NO in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NO antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NO. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The kit should not be used beyond the expiration date.

 

Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
 
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
 
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
 
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Reagent Preparation

All reagents should be brought to room temperature before use.Standard Reconstitute the 120μl of the standard (480μmol/L) with 120μl of standard diluent to generate a 240μmol/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (240μmol/L) 1:2 with standard diluent to produce 120μmol/L, 60μmol/L, 30μmol/L and 15μmol/L solutions. Standard diluent serves as the zero standard(0 μmol/L). Any remaining solution should be frozen at -20℃ and used within one month. Dilution of standard solutions suggested are as follows:

 

240μmol/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
120μmol/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
60μmol/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
30μmol/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
15μmol/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
480μmol/L 240μmol/L 120μmol/L 60μmol/L 30μmol/L 15μmol/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Assay Procedure

1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

4. Add 40μl sample to sample wells and then add 10μl anti-NO antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells ( Not blank control well ). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

Send your inquiry directly to us (0 / 3000)