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Phosphofructokinase Muscle Human ELISA Kit 0.096ng/Ml With 2 Hours Assay Time

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Phosphofructokinase Muscle Human ELISA Kit 0.096ng/Ml With 2 Hours Assay Time

Phosphofructokinase Muscle Human ELISA Kit 0.096ng/Ml With 2 Hours Assay Time
Phosphofructokinase Muscle Human ELISA Kit 0.096ng/Ml With 2 Hours Assay Time

Large Image :  Phosphofructokinase Muscle Human ELISA Kit 0.096ng/Ml With 2 Hours Assay Time

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E6646Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Sensitivity: 0.096ng/ml Quality: CE, ISO
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Discount: Available
Bulk Order: Yes Assay Time: 2 Hours
High Light:

enzyme immunoassay kit

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enzyme assay kits

Human Strong Sensitivity Phosphofructokinase Muscle ELISA Assay Kit PFKM ELISA Test Kit Customized
 
Cat.No E6646Hu
 
Precautions

  • Prior to use, the ELISA Assay Kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent
  • The ELISA Assay Kit should not be used beyond the expiration date.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
 
Reagent Provided

ComponentsQuantity
Standard Solution (80ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (25x)20ml x1
Biotinylated Human PFKM Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 
Standard Curve Range: 0.2ng/ml - 70ng/ml
Sensitivity: 0.096ng/ml
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (80ng/ml) with 120μl of standard diluent to generate a 40ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (40ng/ml) 1:2 with standard diluent to produce 20ng/ml, 10ng/ml, 5ng/ml and 2.5ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
 

40ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
20ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
10ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
5ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
2.5ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
80ng/ml40ng/ml20ng/ml10ng/ml5ng/ml2.5ng/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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