Rabbit Source AKT1 Antibody , Western Blot Use Polyclonal Antibodies

AKT1 Rabbit Polyclonal Antibody

Cat.No. BT-AP00082 Size: 100ul/50ul/20ul Datasheet Request

Product Information

Clonallity: Polyclonal

Applications: WB IHC-p

Source: Rabbit

Immunogen: Synthesized peptide derived from human PDK1 around the non-phosphorylation site of Y9

Protein Name: 3-phosphoinositide-dependent protein kinase 1

Species reactivity: human, mouse, rat

Observed band: 60 kDa

Concentration: 1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification:

The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.

Synonyms:

TNF-alpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF-a; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; Tumour Necrosis Factor Alpha; TNFSF1A; TNFA_HUMAN.

Recommended Dilution:

WB: 1: 500 - 1: 2000

IHC: 1: 100 - 1: 300

​ELISA: 1: 40000

Not yet tested in other applications

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.

Western blot analysis of 1) Hela, 2)3T3, 3) PC12 with Akt1 Rabbit pAb diluted at 1:2,000.. Secondary antibody was diluted at 1:20000​​

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