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Sandwich Enzyme Linked Immunosorbent Assay Test 0.053ng/Ml Sensitivity For Research

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Sandwich Enzyme Linked Immunosorbent Assay Test 0.053ng/Ml Sensitivity For Research

Sandwich Enzyme Linked Immunosorbent Assay Test 0.053ng/Ml Sensitivity For Research
Sandwich Enzyme Linked Immunosorbent Assay Test 0.053ng/Ml Sensitivity For Research

Large Image :  Sandwich Enzyme Linked Immunosorbent Assay Test 0.053ng/Ml Sensitivity For Research

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E2985Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Test Method: Sandwich Standard Curve Rang: 0.1ng/ml - 40ng/ml
Sensitivity: 0.053ng/ml Size: 96 Wells/48 Wells
Custom: Available Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
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elisa reagent kit

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elisa kit

96 Wells Size Human High Precision and Specificity Connexin 43 ELISA Assay Kit For Research

 

Cat.No E2985Hu

 

Precautions

  • Prior to use, the elisa assay kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The elisa assay kit should not be used beyond the expiration date.

 

Intended Use

This elisa assay kit is for the accurate quantitative detection of human Connexin 43 (also known as CX43) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (48ng/ml) with 120μl of standard diluent to generate a 24ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (24ng/ml) 1:2 with standard diluent to produce 12ng/ml, 6ng/ml, 3ng/ml and 1.5ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

24ng/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
12ng/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
6ng/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
3ng/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
1.5ng/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
48ng/ml 24ng/ml 12ng/ml 6ng/ml 3ng/ml 1.5ng/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

 

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

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