Home
Products
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
Company News
Home ProductsBovine ELISA Kits

Sorbitol Dehydrogenase Sandwich Bovine ELISA Kits 96 Wells Plate ISO Approval

China Shanghai Korain Biotech Co., Ltd certification
China Shanghai Korain Biotech Co., Ltd certification
Providing high quality products、perfect after-sales service, and try best to meet the requirement of customers. We are very willing to continue long-term cooperation.

—— Tommy

We have confidence in working with you to expand the market one more year.

—— Lilis

I'm Online Chat Now

Sorbitol Dehydrogenase Sandwich Bovine ELISA Kits 96 Wells Plate ISO Approval

Sorbitol Dehydrogenase Sandwich Bovine ELISA Kits 96 Wells Plate ISO Approval
Sorbitol Dehydrogenase Sandwich Bovine ELISA Kits 96 Wells Plate ISO Approval

Large Image :  Sorbitol Dehydrogenase Sandwich Bovine ELISA Kits 96 Wells Plate ISO Approval

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2015, MSDS
Model Number: Cat.No E0079Bo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Detailed Product Description
Sample: Serum,plasma,urine,tissue,cell Culture Supernatant Quality: CE, ISO
OEM: Acceptable Shipping: DHL/FedEX
Uniprot No.: Q58D31 Custom: Available
High Light:

elisa test kit

,

elisa assay kit

96 Wells Plate Bovine High Sensitivity and Specificity Sorbitol Dehydrogenase Sandwich ELISA Kit
 

Cat.No E0079Bo

Standard Curve Range: 0.5mIU/ml - 100mIU/ml

Sensitivity: 0.27mIU/ml

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Assay Principle

This Sandwich ELISA Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine SDH antibody. SDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine SDH Antibody is added and binds to SDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine SDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (128mIU/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Bovine SDH Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 

Precautions

  • Prior to use, the kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The Sandwich ELISA Kit should not be used beyond the expiration date.

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (128mIU/ml) with 120μl of standard diluent to generate a 64mIU/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (64mIU/ml) 1:2 with standard diluent to produce 32mIU/ml, 16mIU/ml, 8mIU/ml and 4mIU/ml solutions. Standard diluent serves as the zero standard(0 mIU/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

64mIU/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
32mIU/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
16mIU/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
8mIU/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
4mIU/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
128mIU/ml 64mIU/ml 32mIU/ml 16mIU/ml 8mIU/ml 4mIU/ml

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 600 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Assay Procedure

1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

4. Add 40μl sample to sample wells and then add 10μl anti-SDH antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells ( Not blank control well ). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

Send your inquiry directly to us (0 / 3000)