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|Target Protein:||Tumor Necrosis Factor Alpha||Sample:||Serum,plasma,urine,tissue,cell Culture Supernatant|
|Size:||96 Wells/ 48 Wells||Storage:||2-8°C|
enzyme immunoassay kit,
enzyme assay kits
96 wells High specificity Human Tumor Necrosis Factor Alpha ELISA Kit
Standard Curve Range: 3ng/L - 900ng/L
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
This sandwich kit is for the accurate quantitative detection of human Tumor Necrosis Factor Alpha (also known as TNF A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Standard Reconstitute the 120μl of the standard (960ng/L) with 120μl of standard diluent to generate a 480ng/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (480ng/L) 1:2 with standard diluent to produce 240ng/l, 120ng/L, 60ng/L and 30ng/L solutions. Standard diluent serves as the zero standard(0 ng/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
|480ng/L||Standard No.5||120μl Original Standard + 120μl Standard Diluent|
|240ng/L||Standard No.4||120μl Standard No.5 + 120μl Standard Diluent|
|120ng/L||Standard No.3||120μl Standard No.4 + 120μl Standard Diluent|
|60ng/L||Standard No.2||120μl Standard No.3 + 120μl Standard Diluent|
|30ng/L||Standard No.1||120μl Standard No.2 + 120μl Standard Diluent|
|Standard Concentration||Standard No.5||Standard No.4||Standard No.3||Standard No.2||Standard No.1|
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 Shibue T., Tsuchiya N., Komata T., Matsushita M., Shiota M., Ohashi J., Wakui M., Matsuta K., Tokunaga K.
Arthritis Rheum. 43:753-757(2000)
 Nie H., Zheng Y., Li R., Guo T.B., He D., Fang L., Liu X., Xiao L., Chen X., Wan B., Chin Y.E., Zhang J.Z.
Nat. Med. 19:322-328(2013)
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