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Shanghai Korain Biotech Co., Ltd Factory Tour

Good quality Human ELISA Kit for sales
Good quality Human ELISA Kit for sales
Providing high quality products、perfect after-sales service, and try best to meet the requirement of customers. We are very willing to continue long-term cooperation.

—— Tommy

We have confidence in working with you to expand the market one more year.

—— Lilis

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  • Production Line

    Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:

    • ELISA and immunohistochemistry
    • Immunoprecipitation
    • Immunohistochemistry
    • Immunofluorescence microscopy
    • Immunohistochemistry
    • Quantitative Multiplexing

     

    Packing and Shipment

     

    Custom Package

    • different types for you to choose
    • Ice packc and polyfoam material to wrap

     

     

    Ship by DHL/FedEX at our discounted rate

    • Order be shipped within 24 hrs
    • Bulk order shipped within 5 business days

     

     

     

    Shanghai Korain Biotech Co., Ltd                  Shanghai Korain Biotech Co., Ltd

                  Techinical Laboratory                                             Clean Breeding Environment

     

     

  • OEM/ODM

    OEM is also available by us. We can produce as any request from customer.

     

  • R&D

    ELISA Kit Developing Service

    Sandwich/Competitive ELISA kit

    Need to be provided by customers:

    1. Target Proteinor small molecule compound; Possible matched antibody pairs (poly/mono antibody)
    2. Control kit
    3. Relevant technical literatures
    Type QTY Time
    High sensitivity matching selection (Materials provided by customers) One pair (15-20days)
    Normal matching selection (Materials provided by customers) One pair (15-20days)
    Wide detection range matching selection (Materials provided by customers) One pair (15-20days)

    Antibody or Antibody pair can be specifically provided as customer's request.

    Developing charges: 1650USD (Not including the antibody fees)

    Project time:15-20 days
    Developing Results:
    What we provide:
    10 Completed ELISA kits to customer.
    Standard curve report and determination data report.
    Over 10 ELISA kits: 99USD/kit (96T)

     

     

    Antibody Custom Production

    1. Mouse Monoclonal Antibody ProductionProvided by customers

    1) Recombinant purification protein or natural purified protein 5mg(protein purity should be higher than 80%)

    2) Synthetic polypeptide antigen (5mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.

    3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).

    4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.

     

    Producing procedure
    1. Vaccinating 5 BALB/ c strain mice with standard immune procedure. The antibody titers in serum will be determined by ELISA.

    2. After the antibody titers are achieved appropriate values, the mouse spleen cells were fused to form hybridoma. Then determining the supernatant of growing hybridoma cell by ELISA.

    3. Subcloning the hybridoma cell lines by the method of finite dilution, finally provide 2 hybridoma cell lines to the customer.

    4. The Preparation of BALB/ c strain mice Ascites, antibody purification.

    The project completion time: 4-5 months

     

    2.Rabbit Polyclonal Antibody Production

    Provided by customers:
    1) Purification protein or natural purified protein 10mg (protein purity should be higher than 80%).

    2) Prepare polyclonal antibody with polypeptide antigen (10mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.

    3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).

    4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.

    Producing procedure:

    Polypeptide or Protein Antigen/ Mycoprotein, etc. Immunizing 1-2 New Zealand rabbits (or 3 rats); determine the titers by collecting (rabbits) auricular arterial blood, serum were collected by bleeding of carotid artery; determine the titers by collecting (rats) tail veins blood, collected serums from heart.

    Production result

    Plan 1: Provide 10ml serum, titers >1: 10000. Including the report of titers value which were determined by indirect ELISA.
    Plan 2: Povide 10 mg (Rabbit) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, 2 mg (Rat) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, antibody titers(1mg/ml) > 1: 5000; Including the report titers value which were determined by indirect ELISA.
    Plan 3: According to the requirement, collecting serum by sustaining immunization.

    The project completion time: 4-5months.

     

    Antigen Production sevice

    Content(Unit:/each) QTY Purification Time
    Epxress the prokaryotic proteins 10mg >90% 30-45days
    Synthesized and coupled polypeptide 5-10mg/each >90% 30-45days
    Nature purity protein(Provided by customer) 5mg >80% 30-45days
Contact Details
Shanghai Korain Biotech Co., Ltd

Tel: 86-21- 3100 7137

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