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Rabbit Sandwich ELISA Kit , IL 10 ELISA Kit CE / ISO / MSDS Certificated

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Rabbit Sandwich ELISA Kit , IL 10 ELISA Kit CE / ISO / MSDS Certificated

China Rabbit Sandwich ELISA Kit , IL 10 ELISA Kit CE / ISO / MSDS Certificated supplier

Large Image :  Rabbit Sandwich ELISA Kit , IL 10 ELISA Kit CE / ISO / MSDS Certificated

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0004Rb

Payment & Shipping Terms:

Minimum Order Quantity: 1 kit
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
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Detailed Product Description
Target Protein: Interleukin 10 Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
Known As: IL-10 Discount: Available
Customized: Acceptable Assay Principle: Sandwich

Rabbit Interleukin 10 ELISA Kit with High Spesificity and Sensitivity 

Cat.No E0004Rb

Standard Curve Range: 5ng/L - 1500ng/L

Sensitivity: 2.54ng/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit IL-10 antibody. IL-10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit IL-10 Antibody is added and binds to IL-10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit IL-10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (1600ng/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Rabbit IL-10 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

 

800ng/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
400ng/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
200ng/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
100ng/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
50ng/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
1600ng/L 800ng/L 400ng/L 200ng/L 100ng/L 50ng/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

 

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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