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Growth Hormone / GH Bovine ELISA Kits , Sample Detection ELISA Test Kit

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Good quality Human ELISA Kit for sales
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Growth Hormone / GH Bovine ELISA Kits , Sample Detection ELISA Test Kit

China Growth Hormone / GH Bovine ELISA Kits , Sample Detection ELISA Test Kit supplier

Large Image :  Growth Hormone / GH Bovine ELISA Kits , Sample Detection ELISA Test Kit

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0013Bo

Payment & Shipping Terms:

Minimum Order Quantity: 1 kit
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
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Detailed Product Description
Target Protein: Growth Hormone Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
Assay Time: 2 Hours Discount: Available
Uniprot No.: B0FPG6 Cat.No: E0013Bo

Bovine Growth Hormone ELISA Kit with High Spesificity and Sensitivity 
Cat.No E0013Bo
Standard Curve Range: 0.05ng/ml - 10ng/ml
Sensitivity: 0.026ng/ml
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
 
Intended Use
This sandwich kit is for the accurate quantitative detection of Bovine Growth Hormone (also known as GH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
 
Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine GH antibody. GH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine GH Antibody is added and binds to GH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine GH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Reagent Provided

Components Quantity

Standard Solution (12.8ng/ml)

0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Bovine GH Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 
Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
 
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
 
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
 
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (12.8ng/ml) with 120μl of standard diluent to generate a 6.4ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (6.4ng/ml) 1:2 with standard diluent to produce 3.2ng/ml, 1.6ng/ml, 0.8ng/ml and 0.4ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20℃ and used within one month. Dilution of standard solutions suggested are as follows:
 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Assay Procedure
1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.
2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.
3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.
4. Add 40μl sample to sample wells and then add 10μl anti-GH antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells (Not blank control well). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.
5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.
6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.
7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.
8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.
 
References
[1] "A heuristic approach to predicting the tertiary structure of bovine somatotropin."Carlacci L., Chou K.-C., Maggiora G.M.Biochemistry 30:4389-4398(1991)

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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