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High Specifibility and Precision Bovine Vitamin C VC ELISA Kit 96 Wells Size

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High Specifibility and Precision Bovine Vitamin C VC ELISA Kit 96 Wells Size

China High Specifibility and Precision Bovine Vitamin C VC ELISA Kit 96 Wells Size supplier

Large Image :  High Specifibility and Precision Bovine Vitamin C VC ELISA Kit 96 Wells Size

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E2050Bo

Payment & Shipping Terms:

Minimum Order Quantity: 1 kit
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
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Detailed Product Description
OEM: Acceptable Sample: Serum,plasma,urine,tissue,cell Culture Supernatant
Assay Time: 2 Hours Discount: Available
Delivery: Within 48 Hours Assay Principle: Sandwich

Bovine Vitamin C ELISA Kit with High Spesificity and Sensitivity 
Cat.No E2050Bo
Standard Curve Range: 5mg/L - 1800mg/L
Sensitivity: 2.54mg/L
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
 
Intended Use
This sandwich kit is for the accurate quantitative detection of Bovine Vitamin C (also known as VC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
 
Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine VC antibody. VC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine VC Antibody is added and binds to VC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine VC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Reagent Provided

ComponentsQuantity

Standard Solution (640nmol/ml)

0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Bovine VC Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
 
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
 
 

320nmol/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
160nmol/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
80nmol/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
40nmol/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
20nmol/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
640nmol/ml320nmol/ml160nmol/ml80nmol/ml40nmol/ml20nmol/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Assay Procedure
1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.
2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.
3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.
4. Add 40μl sample to sample wells and then add 10μl anti-VC antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells (Not blank control well). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.
5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.
6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.
7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.
8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.
 
 
 

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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