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|Reactivity:||Human, Mouse, Rat, Monkey||Concentration:||1 Mg/ml|
Highly specific PDPK1 Rabbit Polyclonal Antibody
Cat.No. BT-AP00052 Size: 100ul/50ul/20ul Datasheet Request
Immunogen: Synthesized peptide derived from human PDK1 around the non-phosphorylation site of Y9
Protein Name: 3-phosphoinositide-dependent protein kinase 1
Species reactivity: human, mouse, rat, monkey
Observed band: 60 kDa
Concentration: 1 mg/ml
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.
PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1
WB: 1: 500 - 1: 2000
IHC: 1: 200 - 1: 300
Not yet tested in other applications
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases.
PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.
Contact Person: Lee