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Sandwich Mouse ELISA Kit , Mouse Matrix Metalloproteinase 9 ELISA Kit

Good quality Human ELISA Kit for sales
Good quality Human ELISA Kit for sales
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Sandwich Mouse ELISA Kit , Mouse Matrix Metalloproteinase 9 ELISA Kit

China Sandwich Mouse ELISA Kit , Mouse Matrix Metalloproteinase 9 ELISA Kit supplier

Large Image :  Sandwich Mouse ELISA Kit , Mouse Matrix Metalloproteinase 9 ELISA Kit

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0277Mo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
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Detailed Product Description
Uniprot No.: P41245 Target Protein: Matrix Metalloproteinase 9
Assay Length: 2 Hours Lead Time: Within 48 Hours
OEM: Acceptable Assay Principle: Sandwich

High Specificity Mouse MMP-9 ELISA Kit 96 wells
 
Cat.No E0277Mo
Standard Curve Range: 0.05ng/ml - 10ng/ml
Sensitivity: 0.01ng/ml
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
 
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
 
Intended Use
This sandwich kit is for the accurate quantitative detection of Mouse Matrix Metalloproteinase 9 (also known as MMP-9) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
 
Reagent Provided

ComponentsQuantity
Standard Solution (12.8ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Mouse MMP-9 Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (12.8ng/ml) with 120μl of standard diluent to generate a 6.4ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (6.4ng/ml) 1:2 with standard diluent to produce 3.2ng/ml, 1.6ng/ml, 0.8ng/ml and 0.4ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
 

6.4ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
3.2ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
1.6ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
0.8ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
0.4ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
12.8ng/ml6.4ng/ml3.2ng/ml1.6ng/ml0.8ng/ml0.4ng/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary

  1. Prepare all reagents, samples and standards.
  2. Add ample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
  3. Wash the plate 5 times.

  4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

  5. Add stop solution and color develops.

  6. Read the OD value within 10 minutes.

References
Romanic A.M., Harrison S.M., Bao W., Burns-Kurtis C.L., Pickering S., Gu J., Grau E., Mao J., Sathe G.M., Ohlstein E.H., Yue T.L.
Cardiovasc. Res. 54:549-558(2002)

 
 
 
 

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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