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2 Hours Assay Length Rat ELISA Kit for Rat Catalase / CAT Cell Culture Supernates Test

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2 Hours Assay Length Rat ELISA Kit for Rat Catalase / CAT Cell Culture Supernates Test

China 2 Hours Assay Length Rat ELISA Kit for Rat Catalase / CAT Cell Culture Supernates Test supplier

Large Image :  2 Hours Assay Length Rat ELISA Kit for Rat Catalase / CAT Cell Culture Supernates Test

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0869Ra

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
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Detailed Product Description
Uniprot No.: P04762 Size: 96 Wells/48 Wells
OEM: Acceptable Discount: Available
Assay Length: 2 Hours Sample: Serum,plasma,urine,tissue,cell Culture Supernatant

High Specificity ELISA Kit for Catalase CAT 96 wells

 

Cat.No E0869Ra

Standard Curve Range: 1ng/ml - 300ng/ml

Sensitivity: 0.52ng/ml

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

Intended Use

This sandwich kit is for the accurate quantitative detection of Rat Catalase (also known as CAT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Reagent Provided

Components Quantity
Standard Solution (320ng/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Rat CAT Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.

 

Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.

 

Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.

 

Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Summary

  1. Prepare all reagents, samples and standards.
  2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
  3. Wash the plate 5 times.

  4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

  5. Add stop solution and color develops.

  6. Read the OD value within 10 minutes.

Calculation of Result

Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

 

References

Kim C.H., Choi H., Chun Y.S., Kim G.T., Park J.W., Kim M.S.
Pflugers Arch. 442:519-525(2001)

 
 

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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