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Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity

Good quality Human ELISA Kit for sales
Good quality Human ELISA Kit for sales
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Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity

China Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity supplier
Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity supplier Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity supplier

Large Image :  Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0257Mo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Payment Terms: Western Union, T/T
Supply Ability: In Stock
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Detailed Product Description
Delivery: Within 48 Hours Test Method: Sandwich
Lead Time: Within 48 Hours Shipping: DHL/FedEX
Size: 96 Wells/48 Wells Customized: Acceptable

Endothelin 1 Rat ELISA Assay Kit , Mouse ELISA Test Kit With High Precision and Specificity
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Intended Use

This sandwich kit is for the accurate quantitative detection of Mouse Endothelin 1 (also known as ET-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

 

Assay Procedure

1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

4. Add 40μl sample to sample wells and then add 10μl anti-CAMP/LL-37 antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells ( Not blank control well ). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.

 

Assay Principle

This elisa test kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ET-1 antibody. ET-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ET-1 Antibody is added and binds to ET-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ET-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 

Reagent Provided

Components Quantity
Standard Solution (480ng/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Mouse ET-1 Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Precautions

  • Prior to use, the elisa test kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The elisa test kit should not be used beyond the expiration date.

Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
 
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
 
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
 
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.

 

Cat.No E0257Mo

Standard Curve Range: 1ng/L - 400ng/L

Sensitivity: 0.43ng/L

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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