Home
Products
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
Company News
Home ProductsMouse ELISA Kit

HDL Sandwich Mouse Elisa Kit 2-8°C Storage Within 48 Hours Lead Time

Good quality Human ELISA Kit for sales
Good quality Human ELISA Kit for sales
Providing high quality products、perfect after-sales service, and try best to meet the requirement of customers. We are very willing to continue long-term cooperation.

—— Tommy

We have confidence in working with you to expand the market one more year.

—— Lilis

I'm Online Chat Now

HDL Sandwich Mouse Elisa Kit 2-8°C Storage Within 48 Hours Lead Time

China HDL Sandwich Mouse Elisa Kit 2-8°C Storage Within 48 Hours Lead Time supplier

Large Image :  HDL Sandwich Mouse Elisa Kit 2-8°C Storage Within 48 Hours Lead Time

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E0331Mo

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Contact Now
Detailed Product Description
In Stock: Yes Known As: HDL
Storage: 2-8°C Lead Time: Within 48 Hours
Test Method: Sandwich Custom: Available

High Sensitive Mouse High Density Lipoprotein ELISA Kit HDL Sandwich Elisa Kit
 
Cat.No E0331Mo
Standard Curve Range: 0.5ng/ml - 200ng/ml
Sensitivity: 0.26ng/ml
Size: 96 wells
 
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
 
Reagent Provided

ComponentsQuantity
Standard Solution (240ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Mouse HDL Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
 
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
 
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
 
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
 
*Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.
 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (240ng/ml) with 120μl of standard diluent to generate a 120ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (120ng/ml) 1:2 with standard diluent to produce 60ng/ml, 30ng/ml, 15ng/ml and 7.5ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

120ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
60ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
30ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
15ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
7.5ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
240ng/ml120ng/ml60ng/ml30ng/ml15ng/ml7.5ng/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

Send your inquiry directly to us (0 / 3000)