Home
Products
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
Company News
Home ProductsHuman ELISA Kit

48 Wells Human ELISA Kit For Accurate Quantitative Detection Phosphofructokinase Muscle

Good quality Human ELISA Kit for sales
Good quality Human ELISA Kit for sales
Providing high quality products、perfect after-sales service, and try best to meet the requirement of customers. We are very willing to continue long-term cooperation.

—— Tommy

We have confidence in working with you to expand the market one more year.

—— Lilis

I'm Online Chat Now

48 Wells Human ELISA Kit For Accurate Quantitative Detection Phosphofructokinase Muscle

China 48 Wells Human ELISA Kit For Accurate Quantitative Detection Phosphofructokinase Muscle supplier

Large Image :  48 Wells Human ELISA Kit For Accurate Quantitative Detection Phosphofructokinase Muscle

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E6646Hu

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
Contact Now
Detailed Product Description
Sensitivity: 0.096ng/ml Storage: 2-8°C
In Stock: Yes Target Protein: Phosphofructokinase Muscle
Shipping: DHL/FedEX Sample: Serum,plasma,urine,tissue,cell Culture Supernatant

96 Wells 48 Wells Customized Human FKM ELISA Test Kit With High Precision and Specificity
 
Cat.No E6646Hu
 
Reagent Provided

ComponentsQuantity
Standard Solution (80ng/ml)0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (25x)20ml x1
Biotinylated Human PFKM Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

 
Material Required But Not Supplied

  • 37°C±0.5°C incubator
  • Absorbent paper
  • Precision pipettes and disposable pipette tips
  • Clean tubes
  • Deionized or distilled water
  • Microplate reader with 450 ± 10nm wavelength filter

 
Intended Use
This sandwich kit is for the accurate quantitative detection of Human Phosphofructokinase Muscle (also known as PFKM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
 
Assay Principle
This ELISA Assay Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PFKM antibody. PFKM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PFKM Antibody is added and binds to PFKM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PFKM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PFKM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Precautions

  • Prior to use, the ELISA Assay Kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent
  • The ELISA Assay Kit should not be used beyond the expiration date.

 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (80ng/ml) with 120μl of standard diluent to generate a 40ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (40ng/ml) 1:2 with standard diluent to produce 20ng/ml, 10ng/ml, 5ng/ml and 2.5ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
 

40ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
20ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
10ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
5ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
2.5ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

 

Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
80ng/ml40ng/ml20ng/ml10ng/ml5ng/ml2.5ng/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

Send your inquiry directly to us (0 / 3000)