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Rat Strong Sensitivity Elisa Sandwich Test Thiobarbituric Acid Reactive Substance With 2 Hours Assay Time

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Rat Strong Sensitivity Elisa Sandwich Test Thiobarbituric Acid Reactive Substance With 2 Hours Assay Time

China Rat Strong Sensitivity Elisa Sandwich Test Thiobarbituric Acid Reactive Substance With 2 Hours Assay Time supplier

Large Image :  Rat Strong Sensitivity Elisa Sandwich Test Thiobarbituric Acid Reactive Substance With 2 Hours Assay Time

Product Details:

Place of Origin: Shanghai, China
Brand Name: BT Lab
Certification: CE, ISO9001:2005, MSDS
Model Number: Cat.No E1930Ra

Payment & Shipping Terms:

Minimum Order Quantity: Negotiation
Price: Negotiation
Packaging Details: Wrapped with ice pack and styrofoam package
Delivery Time: 1-3 business days, bulk order within one week
Supply Ability: Western Union, T/T
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Detailed Product Description
Assay Time: 2 Hours Known As: TBARS
Storage: 2-8°C Customized: Acceptable
Quality: CE, ISO Sample: Serum,plasma,urine,tissue,cell Culture Supernatant

Rat Strong Sensitivity Thiobarbituric Acid Reactive Substance Sandwich ELISA Kit With 2 Hours Assay Time
 
Cat.No E1369Ra
Standard Curve Range: 0.5nmol/ml - 100nmol/ml
Sensitivity: 0.26nmol/ml
Size: 96 wells
*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
 
Reagent Provided

Components Quantity
Standard Solution (128nmol/ml) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Rat TBARS Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 
Assay Principle
This elisa kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TBARS antibody. TBARS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TBARS Antibody is added and binds to TBARS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TBARS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TBARS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
 
Precautions

  • Prior to use, the elisa kit and sample should be warmed naturally to room temperature 30 minutes.
  • This instruction must be strictly followed in the experiment.
  • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
  • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
  • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
  • Avoid using the reagents from different batches together.
  • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
  • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
  • The elisa kit should not be used beyond the expiration date.

 
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (128nmol/ml) with 120μl of standard diluent to generate a 64nmol/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (64nmol/ml) 1:2 with standard diluent to produce 32nmol/ml, 16nmol/ml, 8nmol/ml and 4nmol/ml solutions. Standard diluent serves as the zero standard(0 nmol/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

64nmol/ml Standard No.5 120μl Original Standard + 120μl Standard Diluent
32nmol/ml Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
16nmol/ml Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
8nmol/ml Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
4nmol/ml Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
128nmol/ml 64nmol/ml 32nmol/ml 16nmol/ml 8nmol/ml 4nmol/ml

 
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
 
Summary
1. Prepare all reagents, samples and standards.
2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.
3. Wash the plate 5 times.
4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.
5. Add stop solution and color develops.
6. Read the OD value within 10 minutes.

Contact Details
Shanghai Korain Biotech Co., Ltd

Contact Person: Lee

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