Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:
Packing and Shipment
Custom Package
Ship by DHL/FedEX at our discounted rate
Techinical Laboratory Clean Breeding Environment
OEM is also available by us. We can produce as any request from customer.
Sandwich/Competitive ELISA kit
Need to be provided by customers:
| Type | QTY | Time |
| High sensitivity matching selection (Materials provided by customers) | One pair | (15-20days) |
| Normal matching selection (Materials provided by customers) | One pair | (15-20days) |
| Wide detection range matching selection (Materials provided by customers) | One pair | (15-20days) |
Antibody or Antibody pair can be specifically provided as customer's request.
Developing charges: 1650USD (Not including the antibody fees)
1) Recombinant purification protein or natural purified protein 5mg(protein purity should be higher than 80%)
2) Synthetic polypeptide antigen (5mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.
3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).
4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.
Producing procedure
1. Vaccinating 5 BALB/ c strain mice with standard immune procedure. The antibody titers in serum will be determined by ELISA.
2. After the antibody titers are achieved appropriate values, the mouse spleen cells were fused to form hybridoma. Then determining the supernatant of growing hybridoma cell by ELISA.
3. Subcloning the hybridoma cell lines by the method of finite dilution, finally provide 2 hybridoma cell lines to the customer.
4. The Preparation of BALB/ c strain mice Ascites, antibody purification.
The project completion time: 4-5 months
2.Rabbit Polyclonal Antibody Production
Provided by customers：
1) Purification protein or natural purified protein 10mg (protein purity should be higher than 80%).
2) Prepare polyclonal antibody with polypeptide antigen (10mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.
3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).
4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.
Producing procedure：
Polypeptide or Protein Antigen/ Mycoprotein, etc. Immunizing 1-2 New Zealand rabbits (or 3 rats); determine the titers by collecting (rabbits) auricular arterial blood, serum were collected by bleeding of carotid artery; determine the titers by collecting (rats) tail veins blood, collected serums from heart.
Production result
Plan 1: Provide 10ml serum, titers >1: 10000. Including the report of titers value which were determined by indirect ELISA.
Plan 2: Povide 10 mg (Rabbit) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, 2 mg (Rat) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, antibody titers(1mg/ml) > 1: 5000; Including the report titers value which were determined by indirect ELISA.
Plan 3: According to the requirement, collecting serum by sustaining immunization.
The project completion time: 4-5months.
Antigen Production sevice
| Content(Unit:/each) | QTY | Purification | Time |
| Epxress the prokaryotic proteins | 10mg | >90% | 30-45days |
| Synthesized and coupled polypeptide | 5-10mg/each | >90% | 30-45days |
| Nature purity protein(Provided by customer) | 5mg | >80% | 30-45days |
1.52ng/L Sensitivity Human TNF Alpha ELISA Kit / Tumor Necrosis Factor Alpha ELISA Kit
48 Wells / 96 Wells Human ELISA Kit , Sandwich Human Leptin ELISA Kit
High Precision Human ELISA Kit , 96 Wells Human Insulin ELISA Kit
High Specificity Rat Catalase ELISA Kit for Accurate Quantitative Detection
Customized Interleukin 1 Alpha Rat ELISA Kit Pre - Coated with Rat IL 1A Antibody
0.052ng/L Sensitivity Interleukin 6 / IL6 Rat ELISA Kit CE / ISO / MSDS Standard
Polyclonal Rabbit Source PDK1 Antibody at -20 °C Storage Temperature
Affinity - Purified ICAM1 Antibody from Rabbit Antiserum Byaffinity - Chromatography
High Sensitive Rabbit Anti DTL Polyclonal Antibodies with 1 mg/ml Concentration
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