Polyclonal Rabbit Source PDK1 Antibody at -20 °C Storage Temperature

Highly specific PDPK1 Rabbit Polyclonal Antibody

Cat.No. BT-AP00052 Size: 100ul/50ul/20ul Datasheet Request

Product Information

Clonallity: Polyclonal

Applications: WB,IHC-p,ELISA

Source: Rabbit

Immunogen: Synthesized peptide derived from human PDK1 around the non-phosphorylation site of Y9

Protein Name: 3-phosphoinositide-dependent protein kinase 1

Species reactivity: human, mouse, rat, monkey

Observed band: 60 kDa

Concentration: 1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification:

The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen.

Synonyms:

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1

Recommended Dilution:

WB: 1: 500 - 1: 2000

IHC: 1: 200 - 1: 300

Not yet tested in other applications

Storage:

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C.​

​Background

PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases.
PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.